Composite

Part:BBa_I712059

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-24)

CMVp - myri - TEVP - T7 RNAP - NLS

Signal sequence for myristoilation, which functions as membrane anchor, fused with proteolytic cleavage site for TEV protease and T7 RNA polymerase with NLS. When assembly of TEV protease occurs, the T7 RNA polymerase is cleaved off the membrane and translocated into nucleus, where the genes under T7 promoter are transcribed.
The CMV promoter is added for constitutive expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None